General Cell Lines and Cell Cultures
Lonza Walkersville Hu NK Cell Nucleofector Kit
The Nucleofector 2b System (or older generations I or II) works cell type specific kits each of them dedicated to an individual primary cell This Nucleofector Kit is the optimal kit for efficient transfection of primary natural killer (NK) cells The respective cell type-specific Optimized Protocol or recommendation is available under Instructions or in our Lonza Bioscience knowledge database In this Optimized Protocol the best Nucleofection conditions are indicated In addition we share our experience and knowledge for treatment of this cell type
Promocell GmbH PromoCell HUVEC Pellet from > 1 million Normal Human Umbilical Vein Endothelial Cells, single donor
Cell pellet consisting of 1 million cells dissolved in 200µl RNAlater for subsequent RNA, DNA or protein analysis. Note: Cell pellets cannot be revived.Our Cell Pellets are stored in RNAlater – an aqueous protection solution that rapidly permeates the cells and stabilizes cellular DNA, RNA, and proteins and protects them from degradation. This makes our Cell Pellets very useful for a wide range of research applications like gene expression analysis, cell characterization studies, and protein characterization experiments.Each Cell Pellet is made from 1 million cells and is dissolved in 200 µl RNAlater. The Cell Pellets are produced from released cell lots and have the same characteristics, i.e. expression profile or cell type specific antigens. On request, viable cells from the same lot are available for cultivation.
Hach Company Ice-Pic 20NTU Cal/Veri Module
Ice-Pic 20 NTUCalibration / Verification ModuleVerify calibration in less than 60 seconds Available in 1 and 20 NTU modules Can be used with the 1720E D and C instruments No loss of sample flow to complete a verification No sample cell to recondition after verification
New England Biolabs, Inc. dam-/dcm- Competent E. coli – 20 x 0.05 ml/tube
Methyltransferase deficient chemically competent E. coli cells suitable for growth of plasmids free of Dam and Dcm methylation. Note that dam- strains are not recommended as a host for primary cloning/ligation. The dam mutation can result in an increased mutation rate in the cell and a reduction in the transformation efficiency. DNA should be maintained in a dam+ strain unless there is a specific need for DNA free of Dam or Dcm methylation.
ATCC CCD-19Lu; Lung Fibroblast; Human (Homo sapiens)
CCD-19Lu is a fibroblast cell line that was isolated from the lung of a normal White, 20-year-old, female patient. This cell line was deposited by S Dilworth.
Biochain Institute Inc Matched Pair - cDNA - Human Primary and Matched Metastatic Tumor Tissue: Colon, 10 x2 rxn /PK
BioChain's human cDNA matched pair products include: Primary Pair (PP), Primary and Metastasis Pair (PM). PP consists of cDNA prepared from primary tumor and its adjacent normal tissue; PM consists of cDNA prepared from primary tumor and corresponding metastatic tumor. cDNAs in each pair are prepared from the same donor. This product line is designed for identifying tumor-specific (or disease specific) genes and tumor metastatic genes.
Biochain Institute Inc Paraffin Tissue Section Panel - Human Adult Normal Tissue, Multi-tissue (8) II: Major Organ, 5 slides/PK
BioChain's human adult normal multiple tissue panels are designed for studying tissue specific cellular localization of genes or proteins of interest in different human normal tissues. Tissues mounted on positively charged glass slides are organized either by organs (major organ multiple tissue panels), or by integrated major systems (organ systems multiple tissue panels). Documentation of tissues' clinical histories is available upon request. Identical format total RNA Northern Blots are available, and cDNA from those tissues may also be available for PCR detection.
Biochain Institute Inc Paraffin Tissue Section - Human Bone Tumor: Chondrosarcoma, Well Differentiated, 5 sides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's formalin fixed paraffin embedded (FFPE) tissue section line encompasses a large selection of tissue sections which are suitable for immunohistochemistry and in situ hybridization assays. The tissue sections are approximately 5 um thick. Each slide contains tissue from a single human tumor. Documentation of tissues' clinical histories may be available upon request. For those customers who want to perform FFPE DNA and RNA isolation, BioChain can provide tissue section slices in tubes instead of mounting them on glass slides.
Enzo Life Sciences LEADING LIGHT Wnt Cell Line Assay Reagents (1 Pack)
For use with the LEADING LIGHT. .The assay has been used successfully in different assay formats (including HTS applications) to identify several distinct categories of small molecule compounds that modulate the Wnt signaling pathway.The LEADING LIGHT.Note: the complete LEADING LIGHT Wnt Reporter Assay Starter Kit (Prod. No. ENZ-61001) is available. Alternative name: Wnt reporter reagents. Shipping: Shipped on Dry Ice. Long Term Storage: -80°C
ATCC Primary Peripheral Blood CD14+ Monocytes, Normal, Human
Primary Peripheral Blood CD14+ Monocytes are primary peripheral blood mononuclear cells isolated from normal blood tissue that have been fully authenticated and characterized by ATCC. These cells can be used in the study of immunology, infection, cancer, hematology, and T-cell suppression assay. One vial contains a minimum of 5.0 × 105 viable cells (provided).
Medchemexpress LLC CD47 protein, rat, recombinant (HEK293), Fc-tagged | 95.0% | 5 UG
Recombinant rat CD47 extracellular domain produced in HEK293 mammalian cells and supplied as an Fc-fusion protein. Designed for in vitro applications including binding assays, ELISA, and cell signaling studies. Available in microgram quantities with estimated purity ~95.0%.
- Mammalian expression ensures native glycosylation and folding.
- Fc fusion facilitates detection, purification, and immobilization.
- Estimated purity of 95.0% supports reliable assay performance.
- Available in small microgram pack sizes for assay optimization.
- Suitable for binding assays, ELISA, and receptor-ligand interaction studies.
ATCC IMR-32; Neuroblastoma; Human (Homo sapiens)
IMR-32 neuroblast cells are isolated from brain tissue that exhibits neuroblastoma. Two cell types are present. Predominant is a small neuroblast-like cell, and the other is a large hyaline fibroblast. Use these to advance your neuroscience research.
Eurofins Discoverx PathHunter NPY2R Activated GPCR Internalization U2OS Cell Line, 2 vials. (93-0721C3)
The PathHunter NPY2R Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of NPY2R via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged NPY2R. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting NPY2R-arrestin complex is internalized to the early endosome, leading to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.